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mcdb170 medium  (US Biological Life Sciences)

 
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    US Biological Life Sciences mcdb170 medium
    Mcdb170 Medium, supplied by US Biological Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mcdb170/human+mammary+epithelial+cell+basal+growth+media+mcdb+170/pm40605016-58-5-9
    Average 90 stars, based on 1 article reviews
    mcdb170 medium - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    other:

    Article Title: AMP-activated protein kinase controls lipid and lactose synthesis in bovine mammary epithelial cells.
    Article Snippet: We purchased MCDB170 from US Biological (Salem, MA).

    Modification:

    Article Title: Neoplastic transformation of porcine mammary epithelial cells in vitro and tumor formation in vivo
    Article Snippet: .. Minced mammary tissue was digested (1.5 mg/ml collagenase A (Roche, San Francisco, CA; 75 μg/ml DNase I, Roche; 1 mg/ml hyaluronidase, MP Biomedicals, Santa Ana, CA) in growth media (10 % fetal bovine serum [FBS], DMEM/F-12, penicillin G/streptomycin sulfate/amphotericin B) at 37 °C for 3 h. Organoids (40–100 μm diameter) were plated in primary porcine mammary epithelial media (modified from MEGM [ ] as a 1:1 mix of MCDB170 (US Biological, Salem, MA) and DMEM/F-12 (CellGro, Manassas, VA) with penicillin G/streptomycin sulfate/amphotericin B, 0.5 % FBS, bovine insulin (7.5 μg/mL, Sigma-Aldrich), human EGF (5 ng/mL, Millipore, Billerica, MA), hydrocortisone (0.25 μg/mL, Sigma-Aldrich), human apo-transferrin (2.5 μg/mL, Sigma-Aldrich), ethanolamine (0.1 mM, Sigma-Aldrich), o-phosphoethanolamine (0.1 mM Sigma-Aldrich), bovine pituitary extract (35 μg/mL, Gemini Bio-Products, West Sacramento, CA), and lipid-rich bovine serum albumin (0.1 %, Gemini Bio-Products). ..



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    ( a ) Primary cell outgrowth, derived from a reduction mammoplasty tissue (RMT) from a 24-year-old woman, cultured in <t>MCDB170</t> medium, and inoculated with pLenti6/CMV-H2B-GFP lentivirus (1 μg ml −1 polybrene). ( b ) Overlay of H2b-GFP signal. ( c ) Overlay of keratin 19 (blue) and keratin 14 (red) immunofluorescence with TO-PRO-3 nuclear counterstain (white). ( d , left) Primary breast cells (passage 1), derived from RMT from a 34-year-old woman, transduced with pLenti6/CMV-ZsGreen lentivirus (+6 μg ml −1 polybrene) and immunostained as in c . ( d , right) Digital removal of red keratin 14 signal; arrowheads mark 3 of the 12 k19 + mitotic cells ( e ) Flow cytometric characterization of primary cells, derived from RMT from a 26-year-old woman (sample N135), cultured in M87 medium and inoculated with pLenti6/CMV-H2B-GFP lentivirus. GFP in transduced cells is compared with the cell expression of lineage markers associated with luminal (Muc1, c-Kit) and basal (CD10, CD49f, Thy1) cell types. ( f ) Quantification of flow cytometry data shown in e . ( g ) Transduction efficiencies of first passage of N135 cells (mixed culture) inoculated with twofold serial dilutions of 1,500 × concentrated CMV-H2B-GFP lentivirus. The fraction of GFP+ cells in the MEPs and LEPs was determined by multi-parameter flow cytometry using Muc1 and Thy1 specific antibodies. The transductional bias has been observed in every (over two dozens) primary culture tested to date. ( a – d ) Scale bars, 100 μm.
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    ( a ) Primary cell outgrowth, derived from a reduction mammoplasty tissue (RMT) from a 24-year-old woman, cultured in MCDB170 medium, and inoculated with pLenti6/CMV-H2B-GFP lentivirus (1 μg ml −1 polybrene). ( b ) Overlay of H2b-GFP signal. ( c ) Overlay of keratin 19 (blue) and keratin 14 (red) immunofluorescence with TO-PRO-3 nuclear counterstain (white). ( d , left) Primary breast cells (passage 1), derived from RMT from a 34-year-old woman, transduced with pLenti6/CMV-ZsGreen lentivirus (+6 μg ml −1 polybrene) and immunostained as in c . ( d , right) Digital removal of red keratin 14 signal; arrowheads mark 3 of the 12 k19 + mitotic cells ( e ) Flow cytometric characterization of primary cells, derived from RMT from a 26-year-old woman (sample N135), cultured in M87 medium and inoculated with pLenti6/CMV-H2B-GFP lentivirus. GFP in transduced cells is compared with the cell expression of lineage markers associated with luminal (Muc1, c-Kit) and basal (CD10, CD49f, Thy1) cell types. ( f ) Quantification of flow cytometry data shown in e . ( g ) Transduction efficiencies of first passage of N135 cells (mixed culture) inoculated with twofold serial dilutions of 1,500 × concentrated CMV-H2B-GFP lentivirus. The fraction of GFP+ cells in the MEPs and LEPs was determined by multi-parameter flow cytometry using Muc1 and Thy1 specific antibodies. The transductional bias has been observed in every (over two dozens) primary culture tested to date. ( a – d ) Scale bars, 100 μm.

    Journal: Nature Communications

    Article Title: Modelling breast cancer requires identification and correction of a critical cell lineage-dependent transduction bias

    doi: 10.1038/ncomms7927

    Figure Lengend Snippet: ( a ) Primary cell outgrowth, derived from a reduction mammoplasty tissue (RMT) from a 24-year-old woman, cultured in MCDB170 medium, and inoculated with pLenti6/CMV-H2B-GFP lentivirus (1 μg ml −1 polybrene). ( b ) Overlay of H2b-GFP signal. ( c ) Overlay of keratin 19 (blue) and keratin 14 (red) immunofluorescence with TO-PRO-3 nuclear counterstain (white). ( d , left) Primary breast cells (passage 1), derived from RMT from a 34-year-old woman, transduced with pLenti6/CMV-ZsGreen lentivirus (+6 μg ml −1 polybrene) and immunostained as in c . ( d , right) Digital removal of red keratin 14 signal; arrowheads mark 3 of the 12 k19 + mitotic cells ( e ) Flow cytometric characterization of primary cells, derived from RMT from a 26-year-old woman (sample N135), cultured in M87 medium and inoculated with pLenti6/CMV-H2B-GFP lentivirus. GFP in transduced cells is compared with the cell expression of lineage markers associated with luminal (Muc1, c-Kit) and basal (CD10, CD49f, Thy1) cell types. ( f ) Quantification of flow cytometry data shown in e . ( g ) Transduction efficiencies of first passage of N135 cells (mixed culture) inoculated with twofold serial dilutions of 1,500 × concentrated CMV-H2B-GFP lentivirus. The fraction of GFP+ cells in the MEPs and LEPs was determined by multi-parameter flow cytometry using Muc1 and Thy1 specific antibodies. The transductional bias has been observed in every (over two dozens) primary culture tested to date. ( a – d ) Scale bars, 100 μm.

    Article Snippet: The resulting divested tissue fragments (organoids) were collected by centrifugation (100 g × 2 min) and either archived in liquid nitrogen (90% FBS+10% dimethylsulphoxide) or immediately placed into culture using serum-free MCDB170 (Lonza) or M87 (M87+CT+X) minimal serum (0.25% FBS) medium , as indicated in the figure legends.

    Techniques: Derivative Assay, Cell Culture, Immunofluorescence, Transduction, Expressing, Flow Cytometry